Dynamic changes of LDH and HBDH activity in rabbit serum after low voltage electrical injury / 法医学杂志

OBJECTIVE@#To investigate changes of LDH and HBDH activity in rabbit serum after non-thermal low voltage electrical injury and to provide diagnostic criteria for non-thermal low voltage electrical injury.@*METHODS@#Forty New Zealand rabbits were randomly distributed into control group and electrical injury group (EI-groups; designated 7 time points: 0 h, 2 h, 4 h, 12 h, 1 d, 2 d, 3 d), 5 rabbits per each group. EI-groups were treated with the method of non-thermal low voltage electrical injury established in our laboratory. Ventricular blood (5 mL) was obtained under anesthesia at designated time points after electrical injury. The activities of LDH and HBDH were measured.@*RESULTS@#Dynamic changes were observed with certain patterns from target serum enzyme activities after electrical injury. Compared with control group, the activities of LDH increased markedly at 4 h, 12 h, and on days 1, 2, and 3 after injury (4 h, 12 h, and day 1 P<0.01; day 2 and day 3 P<0.05). Activities of HBDH increased markedly at day 2 and day 3 after injury (P<0.05). The ratio of HBDH/LDH decreased markedly at 2 h, 4 h, and 12 h after injury (P<0.01).@*CONCLUSION@#Dynamic changes of LDH and HBDH activities may be useful in diagnosis of non-thermal low voltage electrical injury and in estimation of post injury intervals.

Change of myocardial enzymes in patients with acute tetramine poisoning / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the clinical significance of myocardial enzymes in patients with acute tetramine (TEM) poisoning.</p><p><b>METHODS</b>The dynamic changes of the activity of myocardial enzymes and ECG were observed in 67 patients with acute TEM poisoning as well as in 67 healthy people for medical checkup at outpatient department as the control group.</p><p><b>RESULTS</b>Myocardial enzymes of the patients with acute TEM poisoning were increased to different extents on the first day. There was significant difference in the activity of aspartate aminotransferase (AST), creatine phosphokinase (CPK), lactic dehydrogenase (LDH), alpha-hydroxybutyrate dehydrogenase (alpha-HBDH) and CPK isoenzyme MB (CK-MB) between the patients and the healthy people in the control group (P < 0.01). There was significant difference in the myocardial enzymes at different time points among the patients with mild, middle and severe poisoning (P < 0.01). The severer the TEM poisoning was, the higher the activity of myocardial enzymes and the abnormal rate of ECG were. The activity of CPK was the earliest to rise with the highest aptitude of fluctuation and the longest time of elevation. The change of the myocardial enzymes was earlier than that of ECG. The abnormal rate of AST, CPK, CK-MB, LDH, alpha-HBDH was 86.57%, 98.51%, 94.03%, 89.55% and 79.10% respectively. The abnormal rate of ECG was 49.3% on the third day of poisoning. Measurement of myocardial enzymes was better than ECG in sensitivity.</p><p><b>CONCLUSION</b>Acute tetramine poisoning can damage myocardium, which is reversible. CPK is a typical marker for the damage and can serve as one of the important clinical monitoring indices.</p>

Changes of myocardial enzymes in patients with acute carbon monoxide poisoning / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the clinical significance of changes of serum myocardial enzymes in patients with acute carbon monoxide poisoning.</p><p><b>METHODS</b>To determine the dynamic changes of the activity of myocardial enzymes and ECG in 62 patients with acute CO poisoning.</p><p><b>RESULTS</b>In patients with acute CO poisoning 5 kinds of myocardial enzymes begin to increase within 24 hours, the activities of aspartate aminotransferase (AST), creatine phosphokinase (CPK), lactic dehydrogenase (LDH), alpha-hydroxybutyrate dehydrogenase (alpha-HBDH), CPK isoenzyme (CK-MB) were (20.2 +/- 12.3), (151.6 +/- 91.8), (146.8 +/- 50.4), (154.8 +/- 47.7), (13.8 +/- 8.1) U/L respectively, while those in control group were (12.1 +/- 6.7), (90.6 +/- 17.3), (118.7 +/- 13.5), (89.9 +/- 27.9), (5.9 +/- 3.3) U/L respectively. There was significant difference between two groups (P < 0.01); 3 d later, the activities of 5 enzymes were still increased [(21.3 +/- 12.3), (105.8 +/- 51.4), (144.8 +/- 51.4), (159.8 +/- 35.4), (16.2 +/- 9.1) U/L respectively]. 7 and 12 d later, the activities of alpha-HBDH and CK-MB were still higher than those of control (P < 0.01). LDH(1) and LDH(2) increased to peak value in 24 h after poisoning (35.3 +/- 5.8), (43.8 +/- 5.7) U/L vs (24.8 +/- 3.9), (36.9 +/- 4.3) U/L, P < 0.01. The abnormal rate of serum LDH(1) was 78.7%, LDH(2) 58.3%, LDH 45.2%, CK-MB 37.1%, alpha-HBDH 33.6% and the abnormal rate of ECG was less than 10%.</p><p><b>CONCLUSION</b>Acute carbon monoxide poisoning may cause myocardial injury. Determination of serum myocardial enzymes may contribute to showing myocardial injury, early diagnosis and treatment, results of treatment and prognosis.</p>

Acute fluoroacetamide poisoning with main damage to the heart / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To observe the heart damage in 10 patients with acute fluoroacetamide poisoning.</p><p><b>METHODS</b>Monitoring serum activities of myocardial enzymes [creatine kinase (CK), asparate aminotransferase (AST), lactate dehydrogenase(LDH) and hydroxybutyrate dehydrogenase(HBDH)] and recording ECG on these 10 patients were performed during the period of their hospitalization. In the mean while, 24 hour dynamic ECG were also recorded and analysed using GP7000L Holter.</p><p><b>RESULTS</b>(1) Urinary fluorine ion concentrations were increased in 9 patients before therapy and in all these 10 patients during therapeutic period. (2) The activities of serum CK in 2 patients and that of serum HBDH in one patient were increased before therapy. However, the serum activities of one or more than one myocardial enzymes were increased in all these 10 patients during therapeutic period. (3) Four patients had abnormal change of ECG before therapy and 5 patients during therapeutic period. (4) 24 hour dynamic ECG records showed that there were heart electrical alternans in 9 patients. One patient had wandering pacemaker and 6 patients had arythmia.</p><p><b>CONCLUSION</b>Fluoroacetamide may cause obvious heart damage, and also heart electrical alternation.</p>

Correlation between enzymatic estimation of myocardial infarct size and myocardial perfusion defect using SPECT

In patients with acute MI correlation between quantitative estimation of infarct size from the marker enzymes and non enzymatic proteins with perfusion defect using thallium 201 can help in the early choice of management strategy. Concentrations of six-marker enzymes and non-enzymatic protein [CPK, CKMB, LDH, SGOT, alpha HBDH and HFABP] were assayed in serial samples from 12 patients with AMI. Quantitative assessment of infarct size was done by a model developed by Witteveen et al in 1975, a modification was done on the model for estimation of infarct size using HFABP as it overestimates the infarct size using the standard model. Myocardial perfusion defect study using SPECT was done within one week of onset of AMI. Correlation between the infarct size using these enzymes and HFABP with perfusion defect was significant for all except infarct size using peak SGOT. A nearly equal estimate in absolute terms was obtained on using all of total [CPK, LDH, alpha HBDH and HFABP applying the modified model]. Infarct size using CKMB and SGOT underestimates the infarct size, on the other hand infarct size using HFABP is overestimated on applying the standard model. Using peak value of HFABP and applying the modified model can give an early reliable estimate of infarct size within 2 hours of admission or 6 hours of onset of symptoms. Conclusions: Using a single peak value of HFABP at 2 hours from admission or 6 hours from the onset of symptoms and applying the modified model can give an early nearly reliable estimate of infarct size. This can be used for early risk stratification and for the early choice of management strategy

Comparative study on determination of alpha-hydroxybutyrate dehydrogenase, lactate dehydrogenase isoenzyme LDH-1 by adsorption on DEAE Sephadex A-50 & electrophoresis.

Serum alpha-hydroxybutyrate dehydrogenase (HBDH) and lactate dehydrogenase isoenzyme (LDH-1) levels by two techniques viz., adsorption on DEAE Sephadex A-50 and by electrophoresis were estimated in healthy subjects and patients with testicular tumours. The correlation coefficient between HBDH and LDH-1 by adsorption technique was found to be r = 0.8. HBDH was found to have a value almost twice that of LDH-1 by adsorption and electrophoretic technique and the activity of LDH-1 obtained by two methods and of HBDH in normals and patients with testicular tumours could be converted using a factor of 0.557. The quantitative method used for determination of HBDH is reliable, accurate, simple and rapid and therefore has better value in a clinical setting than electrophoresis and adsorption techniques which are laborious and time consuming.

Effects of withdrawal of glucocorticoids on improving the function and enzymatic activities of liver mitochondria in female diabetic rats

En el presente trabajo se describen los efectos de la restitución de corticosterona a ratas con diabetes inducida por estreptozotocina sobre a) la función de mitocondrias enteras de hígado y b) sobre las actividades enzimáticas de la 3- hidroxibutirato deshidrogenasa (HBD), citocromo c oxidasa (Cox) y succinato deshidrogenasa (SD) de mitocondrias que sufrieron ruptura por método físico. La función mitocondrial fue analizadas por la respiración y por el comportamiento oscilatorio osmótico de esas organelas. La respiración se midió por método polarográfico y se midieron el estado 3 de la respiración activa (S3) y el control respiratorio (CR) usando los siguientes sustratos: 3-hidroxibutirato, malato-glutamato y succinato. El comportamiento oscilatorio osmótico se midió usando como parámetro comparativo los coeficientes de amortiguación (CA), que son los cocientes de las amplitudes de dos picos o valles consecutivos obtenidos en el registro espectrofotométrico de dicho fenómeno. Se dispusieron un grupo de ratas normales no diabéticas (N) y los siguientes grupos de ratas diabéticas: controles (D), adrenalectomizadas (D + ADX) y adrenalectomizadas con restitución de corticosterona (D + ADX + C). Los resultados de la respiración mitocondrial mostraron que los valores medios de S3 y CR disminuyeron con los tres sustratos en el grupo D + ADX + C comparado con el grupo D + ADX (p < 0,001). Este grupo demostró, a su vez, un aumento significativo de los valores medios de S3 y CR de respiración con respecto al del grupo D. El comportamiento oscilatorio de mitocondrias enteras de hígado del grupo D + ADX + C demostró un significativo aumento de los CA de picos y valles comparado con los del grupo D + ADX. Los valores de CA del último grupo no fueron significativamente diferentes de los del grupo N. El comportamiento de las actividades enzimáticas de mitocondrias fraccionadas fueron diferentes para cada enzima según los diferentes tratamientos en los grupos de ratas diabéticas. En el grupo D + ADX + C el valor medio de la actividad HBD disminuyó significativamente, el de la Cox aumentó (p < 0,02) y el de la SD no mostró variación alguna con respecto a los correspondientes valores medidos de esas enzimas en el grupo D + ADX. Asimismo, el valor medio de la actividad HBD en este último grupo fue similar al del grupo N y el de Cox fue menor (p < 0,001) que el del grupo D. Se concluye que la corticosterona tiene un significativo efecto diabetogénico sobre la función bioquími

Serum lactate dehydrogenase, alpha-hydroxybutyrate dehydrogenase and ratio of alpha-hydroxybutyrate dehydrogenase to lactate dehydrogenase in testicular tumours.

Serum levels of Lactate Dehydrogenase (LDH), alpha-Hydroxybutyrate Dehydrogenase (HBDH) and the relative ratio of alpha-Hydroxybutyrate Dehydrogenase to Lactate Dehydrogenase (HBDH/LDH) were determined in 51 cases of histologically proved testicular germ cell tumors and 40 healthy controls. Serum LDH and HBDH levels were found to be raised in both seminomatous and non-seminomatous germ cell tumors. The ratio of HBDH/LDH was found to be statistically significant in seminomas and insignificant in non-seminomatous germ cell tumors. Furthermore, the levels of LDH and HBDH showed a fall, following treatment indicating response to therapy. Serum LDH and HBDH could both be used as tumor markers in the diagnosis of testicular germ cell tumors as well as prognostic indicators in monitoring therapy, HBDH, being more specific in monitoring therapy as compared to serum LDH.

Effect in vivo of endogenous sexual hormones upon the liver: mitochondrial function compared in male and female diabetic rats

La diabetes crónica inducida por inyección de estreptozotocina (SZ) después de 30 a 35 días produjo significativas alteraciones de la función mitocondrial del hígado. Se comprobó: 1§) disminución significativa de los valores en el estado 3 (respiración activa con los sustratos 3-hidroxibutirato, malato-glutamato y succinato) y 2§) aumento significativo de los coeficientes de amortiguación de oscilaciones osmóticas. Los parámetros mencionados se midieron en ratas machos y hembras adultas, normales, y los siguientes grupos de ratas diabéticas:) a) machos y hembras enteras, b) machos y hembras gonadectomizados y c) hembras obariectomizadas com restitución de 17 ß-estradiol. Los resultados demonstraron que la ooforectomía de ratas diabéticas provocó aumento significativo de los valores del control respiratorio y del estado 3 de la respiración y disminución de los coeficientes de amortiguación hasta valores cercanos a los de ratas normales no diabéticas. La restitución de 17 ß-estradiol a ratas ooforectomizadas diabéticas volvió ambos parámetros de la función mitocondrial a valores semejantes a los de ratas enteras diabéticas. La orquidectomía de ratas diabéticas no demostró efecto alguno sobre los parámetros mitocondriales ensayados en comparación con machos enteros diabéticos. Se concluye que el endógeno de ratas hembras diabéticas actúa negativamente sobre las alteraciones de la función mitocondrial del hígado en la diabetes experimental crónica

Isoenzyme analyses of Malaysian Schistosoma, S. mekongi and S. japonicum by isoelectric focusing in polyacrylamide gel.

Isoenzyme patterns of adult Malaysian Schistosoma, S. mekongi and S. japonicum strains were analysed by isoelectric focusing (IEF) in polyacrylamide gel. Enzyme patterns obtained from Malaysian Schistosoma homogenates differed from those of S. mekongi and S. japonicum strains. Malaysian Schistosoma was found to differ from S. japonicum by 8 enzymes, namely phosphoglucomutase, phosphoglucoisomerase, malate dehydrogenase, acid phosphatase, hydroxy-butyrate dehydrogenase, hexokinase and alkaline phosphatase, and from S. mekongi by phosphoglucomutase, malate dehydrogenase, aldolase and alkaline phosphatase. These results and the distinct biology of the parasite suggest that Malaysian Schistosoma is a new species in the S. japonicum complex.

Proteção miocárdica em cirurgia cardíaca: estudo comparativo de três métodos / Myocardial protection in heart surgery: comparative study of 3 methods

Foram estudados prospectivamente, com distribuiçäo aleatória, 27 pacientes submetidos a implante de prótese valvular aórtica com circulaçäo extracorpórea, alterando-se o método de proteçäo miocárdica entre a hipotermia local isolada (HL), cardioplegia potássica em soluçäo cristalóide (CC) e cardioplegia potássica em sangue oxigenado (CS). Foram realizadas dosagens das enzimas CPK, DHL, HBDH e TGO da 2ª à 96ª hora de pós-operatório. O grupo submetido à HL (6 casos) apresentou níveis enzímicos no pós-operatório discretamente mais altos em relaçäo aos grupos CC (10 casos) e CS (11 casos). A comparaçäo entre estes 2 grupos evidenciou uma queda mais rápida dos valores enzímicos no pós-operatório do grupo CS. O número de pacientes em classe funcional III-IV (NYHA) näo foi significativamente diferente nos três grupos. Nenhum paciente apresentou níveis séricos de CPK >= 1100 e de TGO >= 80 UI/1. Um paciente do grupo CS (10,0%) e outro no CC (9,1%) necessitaram de suporte farmacológico no pós-operatório, näo sendo as diferenças entre os três grupos significativas. Concluiu-se que a proteçäo miocárdica provida por qualquer dos três métodos foi clinicamente satisfatória, permitindo a análise enzímica distinguir diferença significativa entre os meses em alguns intervalos pós-operatórios

Effects of prolactin and LH on the activity of delta5-3beta hydroxy-steroid dehydrogenase, dihydro-orotic dehydrogenase, b-hydroxybutyrate dehydrogenase and glucose-6-phosphate dehydrogenase in the testis of the dwarf mice.

The activity of delta5-3beta Hydroxysteroid hydro-genase, dihydro orotic dehydrogenase, B-Hydroxybutyrate dehydrogenase and glucose-6-phosphate dehydrogenase was studied in the testes of hereditary dwarf mice treated with Prolactin or LH. Two months old dw/dw dwarf mice were injected twice daily, for 14 days, either with 100 mug ovine Prolactin, or 5 mug ovine LH twice a day. Prolactin treatment increased the activity of all the enzymes assessed. Treatment with LH stimulated the activity of all the enzymes as compared to the saline treated animals but less than the Prolactin treated ones. The data bearing that the increased activity of several oxidising enzymes in the testes of hereditary dwarf mice is increased with Prolactin, is consistent with the suggested effect of this hormone on testicular steroidogenesis.